6V5M
Crystal Structure of Metallo Beta Lactamase from Hirschia baltica in Complex with Succinate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-31 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 77.609, 77.609, 241.883 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.310 - 1.500 |
| R-factor | 0.143 |
| Rwork | 0.142 |
| R-free | 0.16740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6v54 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.129 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 45429 | 2116 |
| <I/σ(I)> | 31.2 | 1 |
| Completeness [%] | 99.7 | 94.1 |
| Redundancy | 14.1 | 4.5 |
| CC(1/2) | 0.494 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.0 M Succinic acid pH 7.0;0.1 M Bis-Tris Propane pH 7.0 |
