6V5F

L-asparaginase from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	273
Detector technology	PIXEL
Collection date	2017-08-10
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.4587
Spacegroup name	P 1 21 1
Unit cell lengths	57.917, 125.435, 68.816
Unit cell angles	90.00, 106.94, 90.00

Refinement procedure

Resolution	33.370 - 2.100
R-factor	0.2325
Rwork	0.230
R-free	0.29760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eca
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (2.8.0)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.100	2.100
Rmeas	0.199	3.401
Number of reflections	105403	16633
<I/σ(I)>	5.38	0.33
Completeness [%]	97.7	95.1
Redundancy	3.15	2.413
CC(1/2)	0.994	0.214

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291.15	0.1 M succinic acid pH 7.0, 15% (w/v) polyethylene glycol 3,350