6V58
Coiled-coil Trimer with Glu:Norleucine:Lys Triad
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-19 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5406 |
| Spacegroup name | H 3 |
| Unit cell lengths | 39.068, 39.068, 113.331 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.780 - 2.201 |
| R-factor | 0.2111 |
| Rwork | 0.204 |
| R-free | 0.27910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uxt |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2906) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.780 | 2.420 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.116 | 0.320 |
| Rmeas | 0.128 | 0.408 |
| Rpim | 0.051 | 0.248 |
| Number of reflections | 3261 | 1210 |
| <I/σ(I)> | 16.2 | 1.7 |
| Completeness [%] | 99.5 | |
| Redundancy | 4.6 | |
| CC(1/2) | 0.977 | 0.796 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 40% v/v PEG600, 100 mM sodium citrate tribasic/citric acid, pH 5.5 |
