6V57

Coiled-coil Trimer with Glu:Norvaline:Lys Triad

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-27
Detector	APEX II CCD
Wavelength(s)	1.5406
Spacegroup name	H 3
Unit cell lengths	38.320, 38.320, 104.585
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.160 - 1.696
R-factor	0.1639
Rwork	0.161
R-free	0.18890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uxt
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHENIX
Refinement software	PHENIX (dev_2906)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.160	1.764
High resolution limit [Å]	1.696	1.696
Rmerge	0.105	0.340
Rmeas	0.111	0.383
Rpim	0.036	0.174
Number of reflections	6357	1233
<I/σ(I)>	17.5	1.9
Completeness [%]	99.8
Redundancy	7.3
CC(1/2)	0.990	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	40% v/v PEG600, 100 mM sodium citrate tribasic/citric acid, pH 5.5