6V55

Full extracellular region of zebrafish Gpr126/Adgrg6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	120
Detector technology	PIXEL
Collection date	2016-04-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033, 0.979
Spacegroup name	C 1 2 1
Unit cell lengths	144.960, 59.448, 168.385
Unit cell angles	90.00, 107.82, 90.00

Refinement procedure

Resolution	47.900 - 2.380
R-factor	0.2152
Rwork	0.212
R-free	0.27170
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.206
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CRANK2
Refinement software	REFMAC (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.900	2.460
High resolution limit [Å]	2.380	2.380
Rmerge	0.064	0.664
Number of reflections	43044	686
<I/σ(I)>	15	0.9
Completeness [%]	77.9	25.4
Redundancy	2.9	1.7
CC(1/2)	0.557	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	50mM potassium dihydrogen phosphate, 20% (w/v) PEG 8000