6V54
Crystal Structure of Metallo Beta Lactamase from Hirschia baltica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 77.668, 77.668, 240.998 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.370 - 1.450 |
| R-factor | 0.1338 |
| Rwork | 0.132 |
| R-free | 0.16250 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.296 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.370 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 49632 | 2422 |
| <I/σ(I)> | 18.4 | 1.7 |
| Completeness [%] | 99.1 | 98.4 |
| Redundancy | 4.1 | 2.8 |
| CC(1/2) | 0.505 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 10% PEG 8000, 0.1M TRIS pH 7.0, 0.2M magnesium chloride |
