6V50
Coiled-coil Trimer with Glu:Ser:Lys Triad with K7A mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-04-02 |
Detector | APEX II CCD |
Wavelength(s) | 1.5406 |
Spacegroup name | H 3 |
Unit cell lengths | 39.190, 39.190, 98.610 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.946 - 2.285 |
R-factor | 0.2028 |
Rwork | 0.197 |
R-free | 0.26570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ovs |
RMSD bond length | 0.006 |
RMSD bond angle | 0.677 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.946 | 2.615 |
High resolution limit [Å] | 2.285 | 2.285 |
Rmerge | 0.100 | 0.354 |
Rmeas | 0.104 | 0.104 |
Rpim | 0.029 | 0.135 |
Number of reflections | 5113 | 258 |
<I/σ(I)> | 12 | 2.3 |
Completeness [%] | 99.9 | |
Redundancy | 12.6 | |
CC(1/2) | 0.999 | 0.946 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 40% PEG300, 100 mM HEPES/NaOH, pH 7.5, 200 mM sodium chloride |