6V4Y
Coiled-coil Trimer with Glu:Tyr:Lys Triad with a K7A mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-07-11 |
Detector | APEX II CCD |
Wavelength(s) | 1.5406 |
Spacegroup name | H 3 |
Unit cell lengths | 39.814, 39.814, 98.199 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.907 - 1.850 |
R-factor | 0.221 |
Rwork | 0.218 |
R-free | 0.24680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ovs |
RMSD bond length | 0.006 |
RMSD bond angle | 0.732 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.907 | 1.948 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.199 | 0.450 |
Rmeas | 0.206 | 0.523 |
Rpim | 0.052 | 0.261 |
Number of reflections | 9078 | 990 |
<I/σ(I)> | 17.5 | 1.1 |
Completeness [%] | 99.8 | |
Redundancy | 9.4 | |
CC(1/2) | 0.913 | 0.822 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 35% 2-ethoxyethanol, 100 mM imidazole/HCl, pH 8, 50 mM calcium acetate |