6V4M
Trichuris suis BCL-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 55.248, 55.248, 111.249 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.199 - 1.599 |
R-factor | 0.17 |
Rwork | 0.169 |
R-free | 0.19160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3FDL Chain A |
RMSD bond length | 0.006 |
RMSD bond angle | 0.774 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.11_2567) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.199 | 1.700 |
High resolution limit [Å] | 1.599 | 1.600 |
Rmerge | 0.078 | 0.760 |
Number of reflections | 23576 | 3723 |
<I/σ(I)> | 25.15 | 3.94 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.1 | 14.2 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-chloride pH8.5, 0.2M Lithium sulphate, 2M sodium chloride |