6V4M
Trichuris suis BCL-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 55.248, 55.248, 111.249 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.199 - 1.599 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.19160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3FDL Chain A |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.774 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX (1.11_2567) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.199 | 1.700 |
| High resolution limit [Å] | 1.599 | 1.600 |
| Rmerge | 0.078 | 0.760 |
| Number of reflections | 23576 | 3723 |
| <I/σ(I)> | 25.15 | 3.94 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.1 | 14.2 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-chloride pH8.5, 0.2M Lithium sulphate, 2M sodium chloride |
