6V4F
Crystal Structure Analysis of Zebra Fish MDMX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.140, 108.160, 30.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.570 - 1.350 |
| R-factor | 0.1696 |
| Rwork | 0.169 |
| R-free | 0.18990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n5t |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.810 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.570 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmeas | 0.049 | 1.580 |
| Rpim | 0.021 | 0.663 |
| Total number of observations | 162828 | 8088 |
| Number of reflections | 30039 | 1474 |
| <I/σ(I)> | 15.4 | 1.1 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100mM HEPES-7.0, 1.6M ammonium sulfate |
