6V4C
Culex quinquefasciatus D7 long form 1- CxD7L1 in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2019-10-19 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0000 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 76.660, 84.320, 132.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.020 - 1.970 |
R-factor | 0.2151 |
Rwork | 0.214 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nht |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.020 | 39.020 | 2.020 |
High resolution limit [Å] | 1.970 | 8.810 | 1.970 |
Rmerge | 0.066 | 0.038 | 0.643 |
Rmeas | 0.072 | 0.042 | 0.705 |
Total number of observations | 179520 | ||
Number of reflections | 30364 | 332 | 2235 |
<I/σ(I)> | 12.19 | 19.71 | 2.35 |
Completeness [%] | 99.1 | 83.6 | 100 |
Redundancy | 5.912 | 5 | 5.948 |
CC(1/2) | 0.998 | 0.998 | 0.839 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES monohydrate, pH 6.5, 25% 550 PEG MME, 0.01 M Zn sulfate |