6V4B
DeCLIC N-terminal Domain 34-202
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2018-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 61 |
| Unit cell lengths | 81.350, 81.350, 99.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.840 - 1.750 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.22000 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.750 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.060 | 1.210 |
| Number of reflections | 37644 | 2724 |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.4 | |
| CC(1/2) | 0.999 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES pH 6.5, 0.7 M NaH2PO4, 0.7 M KH2PO4. |
