6V44
The crystal structure of hemagglutinin from swine influenza virus A/swine/Missouri/A01727926/2015
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-13 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.973, 240.005, 68.953 |
| Unit cell angles | 90.00, 119.86, 90.00 |
Refinement procedure
| Resolution | 33.207 - 2.200 |
| R-factor | 0.2051 |
| Rwork | 0.203 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xl2 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 95268 | 8895 |
| <I/σ(I)> | 29.5 | |
| Completeness [%] | 98.0 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 0.1M Tris-HCl, pH8.5, 30% (w/v) PEG 1000 |
