6V42
Crystal structure of the flavin oxygenase with cofactor bound involved in folate catabolism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9776 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.669, 125.209, 127.329 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.740 - 1.450 |
| R-factor | 0.1534 |
| Rwork | 0.152 |
| R-free | 0.17550 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.998 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.750 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.063 | |
| Rmeas | 0.068 | |
| Number of reflections | 173949 | 8569 |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M TRIS hydrochloride pH 8.5, 2.0M ammonium sulfate |
