6V3J
KIR3DL1 in complex with HLA-B*57:03 presenting the peptide LSSPVTKSF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.759, 60.771, 65.360 |
| Unit cell angles | 96.65, 97.39, 109.18 |
Refinement procedure
| Resolution | 48.107 - 1.980 |
| R-factor | 0.1928 |
| Rwork | 0.191 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vh8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 1.975 | 1.980 |
| Rmerge | 0.076 | 0.480 |
| Number of reflections | 50170 | 7050 |
| <I/σ(I)> | 10.3 | 2.5 |
| Completeness [%] | 97.3 | 93.7 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 14% PEG 3350, 2% tacsimate, pH 5.0, 0.1 M tri-sodium citrate pH 5.6 |
