6V36
K2P2.1(TREK-1)I110D apo channel structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.192, 120.400, 128.355 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.980 - 3.400 |
R-factor | 0.2876 |
Rwork | 0.286 |
R-free | 0.31790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rue |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.700 | 3.670 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 14468 | 1096 |
<I/σ(I)> | 9.6 | |
Completeness [%] | 99.9 | |
Redundancy | 6.5 | |
CC(1/2) | 0.999 | 0.128 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M KCl, 1-3 mM CdCl2, HEPES pH 7-8, 20-26% PEG400 |