6V36

K2P2.1(TREK-1)I110D apo channel structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	68.192, 120.400, 128.355
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.980 - 3.400
R-factor	0.2876
Rwork	0.286
R-free	0.31790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rue
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.700	3.670
High resolution limit [Å]	3.400	3.400
Number of reflections	14468	1096
<I/σ(I)>	9.6
Completeness [%]	99.9
Redundancy	6.5
CC(1/2)	0.999	0.128

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M KCl, 1-3 mM CdCl2, HEPES pH 7-8, 20-26% PEG400