6V33
X-ray structure of a sugar N-formyltransferase from Pseudomonas congelans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-03-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.98987 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 90.364, 90.364, 124.647 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.600 - 2.030 |
| R-factor | 0.2025 |
| Rwork | 0.201 |
| R-free | 0.22130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yfy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.747 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.130 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 36719 | 5131 |
| <I/σ(I)> | 51.6 | 3.6 |
| Completeness [%] | 95.1 | 93.7 |
| Redundancy | 6.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 16-19% PEG-5000, 200 mM LiCl, 5 mM TDP-Qui4N, 5 mM folonic acid |
