6V23
Ligand-free L-aspraginase II from E. coli (EcAII)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-04 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 62.028, 71.192, 130.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.440 - 1.750 |
R-factor | 0.185 |
Rwork | 0.182 |
R-free | 0.24130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eca |
RMSD bond length | 0.019 |
RMSD bond angle | 2.296 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.780 |
High resolution limit [Å] | 1.750 | 4.750 | 1.750 |
Rmerge | 0.046 | 0.037 | 0.489 |
Rmeas | 0.050 | 0.040 | 0.591 |
Rpim | 0.020 | 0.016 | 0.325 |
Total number of observations | 162946 | ||
Number of reflections | 27916 | 1567 | 1018 |
<I/σ(I)> | 19 | ||
Completeness [%] | 95.3 | 99.1 | 70.1 |
Redundancy | 5.8 | 6.2 | 2.8 |
CC(1/2) | 0.998 | 0.678 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 0.1 M magnesium formate and 16% (w/v) PEG3350 |