6V1V

VIP3B (VIP3B_2160) adapted for crystallization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-16
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	106.488, 106.542, 117.728
Unit cell angles	96.13, 70.93, 69.52

Refinement procedure

Resolution	49.892 - 3.189
Rwork	0.218
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	truncated protein (structure to be published)
RMSD bond length	0.009
RMSD bond angle	1.571
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.892	3.260
High resolution limit [Å]	3.189	3.189
Rmerge	0.105	0.950
Rmeas	0.117	0.950
Rpim	0.064	0.668
Number of reflections	72704	3617
<I/σ(I)>	15.2
Completeness [%]	98.7	98.9
Redundancy	3.9	3.5
CC(1/2)		0.388

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	25% PEG 1500, 100 mM Bis-Tris Propane pH 9.0, 100 mM NaCl.