6V1V
VIP3B (VIP3B_2160) adapted for crystallization
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-16 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 106.488, 106.542, 117.728 |
Unit cell angles | 96.13, 70.93, 69.52 |
Refinement procedure
Resolution | 49.892 - 3.189 |
Rwork | 0.218 |
R-free | 0.25860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | truncated protein (structure to be published) |
RMSD bond length | 0.009 |
RMSD bond angle | 1.571 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.892 | 3.260 |
High resolution limit [Å] | 3.189 | 3.189 |
Rmerge | 0.105 | 0.950 |
Rmeas | 0.117 | 0.950 |
Rpim | 0.064 | 0.668 |
Number of reflections | 72704 | 3617 |
<I/σ(I)> | 15.2 | |
Completeness [%] | 98.7 | 98.9 |
Redundancy | 3.9 | 3.5 |
CC(1/2) | 0.388 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 25% PEG 1500, 100 mM Bis-Tris Propane pH 9.0, 100 mM NaCl. |