6V1R
Crystal structure of iAChSnFR Fluorescent Acetylcholine Sensor precursor binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.760, 65.781, 96.592 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.110 - 1.640 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6efr |
RMSD bond length | 0.006 |
RMSD bond angle | 0.863 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.110 | 1.704 |
High resolution limit [Å] | 1.640 | 1.645 |
Rmerge | 0.069 | 1.257 |
Rmeas | 0.076 | 1.366 |
Rpim | 0.030 | 0.527 |
Number of reflections | 36549 | 3406 |
<I/σ(I)> | 13.18 | 1.17 |
Completeness [%] | 98.9 | 93.98 |
Redundancy | 6.4 | 6.3 |
CC(1/2) | 0.999 | 0.636 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 20% PEG 8,000 0.1 M CHES, pH 9.5 10 mM spermidine |