EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | DIRECT ELECTRON DE-64 (8k x 8k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.98 |
Symmetry | I |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |