6V1A
immune receptor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.344, 56.990, 131.184 |
| Unit cell angles | 90.00, 90.87, 90.00 |
Refinement procedure
| Resolution | 43.720 - 2.290 |
| R-factor | 0.1882 |
| Rwork | 0.187 |
| R-free | 0.21370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bil |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.639 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.240 | 2.360 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.104 | |
| Number of reflections | 49925 | 4179 |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 99.1 | 91.1 |
| Redundancy | 6.9 | |
| CC(1/2) | 0.997 | 0.812 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M trisodium citrate, 20% PEG 3350 |
