6V13

immune receptor complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	150.457, 56.766, 136.253
Unit cell angles	90.00, 92.42, 90.00

Refinement procedure

Resolution	45.380 - 2.750
R-factor	0.2115
Rwork	0.210
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bil
RMSD bond length	0.012
RMSD bond angle	0.943
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.380	2.900
High resolution limit [Å]	2.750	2.750
Rmerge		1.342
Number of reflections	30349	4424
<I/σ(I)>	9.4
Completeness [%]	99.9
Redundancy	7
CC(1/2)	0.993	0.587

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M trisodium citrate, 20% PEG 3350