6V0T

Crystal Structure of Catalytic Subunit of Bovine Pyruvate Dehydrogenase Phosphatase 1 - Catalytic Domain

Replaces:  3N3C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-18
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97900
Spacegroup name	P 32 2 1
Unit cell lengths	75.339, 75.339, 173.255
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.117 - 2.100
R-factor	0.221
Rwork	0.219
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a6q
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.117	2.155
High resolution limit [Å]	2.100	2.100
Number of reflections	33626	2014
<I/σ(I)>	35.3
Completeness [%]	96.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	298	The protein solution was 20mg/mL in 10 mM Tris-HCl, pH 7.3, 50 mM NaCl, 1mM MnSO4, 2mM DTT, and 1mM benzamide. The well solution (mixed 1:1) contained 0.10M HEPES, pH 7.5 and 20% Jeffamine M-600., Hanging drop, temperature 298K