6V0M
Sterile alpha-motif from apoptosis signal-regulating kinase 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537, 1.456 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.415, 53.558, 96.258 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.130 - 1.800 |
R-factor | 0.22145 |
Rwork | 0.219 |
R-free | 0.26307 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.657 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.130 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.053 | 0.985 |
Rpim | 0.044 | 0.787 |
Number of reflections | 22425 | 1305 |
<I/σ(I)> | 11.5 | |
Completeness [%] | 99.4 | |
Redundancy | 4.2 | |
CC(1/2) | 0.998 | 0.456 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 290 | 0.1 M sodium citrate tribasic dihydrate pH 5, 25 % (w/v) PEG3350 |