6V04
DynU16 crystal structure, a putative protein in the dynemicin biosynthetic locus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-15 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 73.171, 73.171, 123.944 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.610 - 1.500 |
R-factor | 0.1631 |
Rwork | 0.162 |
R-free | 0.18820 |
Structure solution method | SIRAS |
RMSD bond length | 0.005 |
RMSD bond angle | 0.831 |
Data reduction software | XDS (Nov 11, 2017) |
Data scaling software | XDS (Nov 11, 2017) |
Phasing software | SHELX (2016/1) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.310 | 44.310 | 1.600 |
High resolution limit [Å] | 1.500 | 4.490 | 1.500 |
Rmerge | 0.042 | 0.031 | 0.715 |
Rmeas | 0.044 | 0.033 | 0.806 |
Number of reflections | 112506 | 4413 | 13993 |
<I/σ(I)> | 23.16 | 69.78 | 1.39 |
Completeness [%] | 95.3 | 99.4 | 73.1 |
Redundancy | 8.974 | 10.246 | 4.637 |
CC(1/2) | 1.000 | 0.999 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1ul:1ul, 0.1M Tris-HCl pH8.5, 0.2M MgCl2, 20% PEG 8000 |