6V02
N-terminal 5 domains of CI-MPR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-29 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.780, 66.220, 124.530 |
| Unit cell angles | 90.00, 100.53, 90.00 |
Refinement procedure
| Resolution | 35.910 - 2.460 |
| R-factor | 0.24356 |
| Rwork | 0.240 |
| R-free | 0.30778 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6p8i |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.215 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 122.432 | 2.760 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Number of reflections | 19610 | 981 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 91.1 | 58.2 |
| Redundancy | 5 | 3.9 |
| CC(1/2) | 0.999 | 0.605 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292.15 | 100 mM HEPES pH 7.0, 30% Jeffamine ED-2003 |
