Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.152, 41.364, 71.933 |
| Unit cell angles | 90.00, 103.83, 90.00 |
Refinement procedure
| Resolution | 29.094 - 1.500 |
| R-factor | 0.1623 |
| Rwork | 0.161 |
| R-free | 0.18420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ks3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.316 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10pre_2097) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.058 | 0.046 | 0.347 |
| Rmeas | 0.063 | 0.049 | 0.401 |
| Rpim | 0.023 | 0.018 | 0.197 |
| Number of reflections | 65546 | 2032 | 1555 |
| <I/σ(I)> | 11.9 | ||
| Completeness [%] | 97.6 | 99.6 | 80.3 |
| Redundancy | 6.8 | 7.2 | 3.9 |
| CC(1/2) | 0.998 | 0.871 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.6 M Na-Citrate, 50 mM Tris, pH 7.8 |
