6UYZ

Crystal structure of K46-acetylated SUMO1 in complex with phosphorylated DAXX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	100.224, 38.316, 67.721
Unit cell angles	90.00, 119.91, 90.00

Refinement procedure

Resolution	48.280 - 1.400
R-factor	0.16
Rwork	0.159
R-free	0.18100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wjn
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.275	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	41097	2602
<I/σ(I)>	10.93
Completeness [%]	92.8
Redundancy	3
CC(1/2)	0.997	0.428

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100MM SODIUM CACODYLATE PH6.5, 26% PEG3350, 10MM CALCIUM CHLORIDE