6UYF
Structure of Hepatitis C Virus Envelope Glycoprotein E2mc3-v1 redesigned core from genotype 6a bound to broadly neutralizing antibody AR3B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 175.214, 54.040, 71.486 |
Unit cell angles | 90.00, 94.74, 90.00 |
Refinement procedure
Resolution | 43.650 - 2.060 |
R-factor | 0.1907 |
Rwork | 0.189 |
R-free | 0.23150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bkc |
RMSD bond length | 0.004 |
RMSD bond angle | 0.990 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.060 | 2.060 |
Number of reflections | 38846 | 1408 |
<I/σ(I)> | 15.9 | |
Completeness [%] | 93.9 | |
Redundancy | 4.6 | |
CC(1/2) | 0.960 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG 8000, 0.1M HEPES pH=7.5 |