6UY1

Crystal structure of the Sth1 bromodomain from Saccharomyces cerevisiae at 2.2 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	76.279, 76.281, 98.443
Unit cell angles	90.00, 96.65, 90.00

Refinement procedure

Resolution	19.950 - 2.210
R-factor	0.1856
Rwork	0.184
R-free	0.22800
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.280
High resolution limit [Å]	2.200	4.720	2.200
Rmerge	0.095	0.057	0.633
Total number of observations	749157
Number of reflections	108351	10947	10242
<I/σ(I)>	8.8
Completeness [%]	98.9	99.9	93.3
Redundancy	6.9	7.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1 M BIS-TRIS pH 6.5 0.2 M magnesium chloride hexahydrate 25% (w/v) Polyethylene glycol 3350