6UXT

Crystal structure of unknown function protein yfdX from Shigella flexneri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-11
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	51.579, 92.920, 93.469
Unit cell angles	97.35, 98.44, 95.46

Refinement procedure

Resolution	43.517 - 1.797
R-factor	0.1716
Rwork	0.170
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a02
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.797	4.880	1.800
Rmerge	0.062	0.045	0.477
Rmeas	0.073	0.053	0.562
Rpim	0.037	0.027	0.296
Number of reflections	143645	7486	7183
<I/σ(I)>	7.1
Completeness [%]	91.4	95.3	91.4
Redundancy	3.7	3.8	3.5
CC(1/2)		0.997	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	297	20% PEG3350, 0.2M ammonium citrate, 50mM MES