6UXK

Structure of serine hydroxymethyltransferase 8 from Glycine max cultivar Forrest complexed with PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-08-23
Detector	RDI CMOS_8M
Wavelength(s)	1.00
Spacegroup name	P 2 21 21
Unit cell lengths	56.120, 127.970, 128.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.696 - 2.100
R-factor	0.1835
Rwork	0.181
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uxh
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.100	2.828
Rmeas	0.103	2.937
Rpim	0.023	0.760
Total number of observations	1094603	62685
Number of reflections	54674	4402
<I/σ(I)>	26.4	0.8
Completeness [%]	99.9	99.8
Redundancy	20	14.2
CC(1/2)	1.000	0.392

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.21 M trimethylamine N-oxide, 0.1 M tris pH 8.5, 20% (w/v) PEG MME 2000