6UXC

1.65A resolution structure of the hypothetical protein CT253 from Chlamydia trachomatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 31 2 1
Unit cell lengths	80.187, 80.187, 114.841
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.720 - 1.650
R-factor	0.1729
Rwork	0.171
R-free	0.20390
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.17.1_3678)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.250	44.250	1.680
High resolution limit [Å]	1.650	9.040	1.650
Rmerge	0.049	0.024	1.525
Total number of observations	487014	3426	24989
Number of reflections	51872	378	2562
<I/σ(I)>	20.2	68.5	1.6
Completeness [%]	99.7	99.4	100
Redundancy	9.4	9.1	9.8
CC(1/2)	1.000	0.999	0.647

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	2 M sodium formate, 0.1M Tris