6UWJ
Intermediate engineered variant of I-OnuI meganuclease with improved thermostability and partially altered specificity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 108 |
| Detector technology | PIXEL |
| Collection date | 2019-04-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.288, 62.586, 157.405 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.985 - 1.852 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.23420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qqy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.136 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.8.1) |
| Refinement software | PHENIX (1.13) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
| Rmerge | 0.035 | 0.027 | 0.162 |
| Rmeas | 0.037 | 0.028 | 0.175 |
| Rpim | 0.011 | 0.008 | 0.063 |
| Total number of observations | 374757 | ||
| Number of reflections | 33122 | 3578 | 3166 |
| <I/σ(I)> | 20.2 | ||
| Completeness [%] | 99.7 | 99.9 | 96.7 |
| Redundancy | 11.3 | 11.6 | 7.3 |
| CC(1/2) | 0.999 | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM sodium acetate pH 6.0, 200mM calcium acetate, 35% PEG 400 |
