6UWA
Mouse PKC C1B and C2 domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2012-02-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.94967 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.041, 58.974, 104.954 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.390 - 1.200 |
| R-factor | 0.1348 |
| Rwork | 0.134 |
| R-free | 0.15340 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.083 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_3126) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.203 | 1.214 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.133 | 0.718 |
| Rpim | 0.054 | 0.348 |
| Number of reflections | 126177 | 3798 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 98.2 | |
| Redundancy | 5.3 | |
| CC(1/2) | 0.996 | 0.658 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289.15 | 14% PEG 3350, 0.3 mM Lithium sulfate, 50 mM Ammonium citrate tribasic, 0.1 M HEPES pH 7.0 |
