6UW3

The crystal structure of FbiA from Mycobacterium Smegmatis, GDP Bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	46.067, 73.629, 90.931
Unit cell angles	90.00, 95.38, 90.00

Refinement procedure

Resolution	45.864 - 2.400
R-factor	0.1959
Rwork	0.193
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c3d
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.070	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.139	0.740
Rpim	0.106	0.644
Number of reflections	23704	2453
<I/σ(I)>	6.7	1.7
Completeness [%]	99.6
Redundancy	3.3
CC(1/2)	0.986	0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	18 % PEG6000, 0.1 M Tris, 0.2 M calcium acetate