6UVO
Structure of antibody 3G12 bound to the central conserved domain of RSV G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.11503 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 139.330, 139.330, 94.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 74.530 - 2.900 |
| R-factor | 0.1943 |
| Rwork | 0.193 |
| R-free | 0.20880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k59 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.067 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 74.531 | 74.530 | 3.080 |
| High resolution limit [Å] | 2.900 | 8.700 | 2.900 |
| Rmerge | 0.109 | 0.037 | 0.732 |
| Rmeas | 0.126 | 0.043 | 0.847 |
| Rpim | 0.062 | 0.022 | 0.419 |
| Total number of observations | 3561 | 14475 | |
| Number of reflections | 23682 | 934 | 3763 |
| <I/σ(I)> | 9.4 | 21.1 | 1.9 |
| Completeness [%] | 99.5 | 98.4 | 99.5 |
| Redundancy | 3.9 | 3.8 | 3.8 |
| CC(1/2) | 0.993 | 0.997 | 0.601 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.4 | 277.15 | 1.8M Ammonium Sulfate, 100mM sodium acetate trihydrate pH4.4 |
