6UVI
Crystal structure of alr1298, a pentapeptide repeat protein from Nostoc Pcc 7120, determined at 2.3 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97931 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.762, 85.922, 95.686 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.320 - 2.100 |
R-factor | 0.2144 |
Rwork | 0.208 |
R-free | 0.27440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uv7 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.196 |
Data reduction software | XDS (1.1.7) |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHENIX (1.13_2998) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.320 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.019 | 0.065 |
Rmeas | 0.026 | 0.071 |
Rpim | 0.019 | 0.028 |
Number of reflections | 22287 | 22336 |
<I/σ(I)> | 18.53 | 17.7 |
Completeness [%] | 98.0 | |
Redundancy | 2 | |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% w/v Polyethylene glycol 4,000 |