6UVB
Crystal structure of far-red-light absorbing cyanobacteriochrome at 100K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-05 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 108.788, 108.788, 68.697 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.703 - 3.000 |
| R-factor | 0.2389 |
| Rwork | 0.236 |
| R-free | 0.28970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4glq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.493 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 7.860 | 2.900 |
| Rmerge | 0.080 | 0.039 | 1.688 |
| Rmeas | 0.087 | 0.042 | 1.834 |
| Rpim | 0.033 | 0.016 | 0.705 |
| Total number of observations | 63276 | ||
| Number of reflections | 9394 | 536 | 456 |
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 98.9 | 97.1 | 100 |
| Redundancy | 6.7 | 6.3 | 6.5 |
| CC(1/2) | 0.998 | 0.488 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 288 | The protein solution (3 mg/ml) is mixed with the crystallization solution (17% PEG 10000, 0.1 M ammonium acetate, 0.1 M Bis-tris pH 5.5 with Yttrium(III) chloride as an additive) in a 1:1 ratio |
