6UV8
Crystal structure of a far-red cyanobacteriochrome photoreceptor at room temperature
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 297 |
Detector technology | CCD |
Collection date | 2018-12-12 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.98-1.2 |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 108.800, 108.800, 67.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.657 - 2.701 |
R-factor | 0.1824 |
Rwork | 0.180 |
R-free | 0.23780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4glq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.256 |
Data reduction software | Precognition |
Phasing software | PHENIX (1.10.1_2155) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.790 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.038 | 0.746 |
Number of reflections | 8453 | 397 |
<I/σ(I)> | 43.4 | 1.3 |
Completeness [%] | 98.7 | 99.8 |
Redundancy | 10.3 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 5.5 | 297 | 2 mg/mL protein, 8.5-10.5% polyethylene glycol 10,000 (PEG), 50 mM NH4CH3COO, and 50 mM Bis-tris buffer at pH 5.5 |