6UV7
Crystal structure of alr1298, a pentapeptide repeat protein from Nostoc Pcc 7120, determined at 2.3 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97931 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.842, 86.363, 96.047 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.020 - 2.270 |
R-factor | 0.2043 |
Rwork | 0.199 |
R-free | 0.25090 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.288 |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHENIX (1.13_2998) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.020 | 2.400 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.028 | |
Rmeas | 0.039 | |
Rpim | 0.028 | |
Number of reflections | 18106 | 18173 |
<I/σ(I)> | 17.18 | |
Completeness [%] | 99.6 | |
Redundancy | 2 | 12.6 |
CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% w/v Polyethylene glycol 4,000 |