6UV4

Crystal structure of the core domain of RNA helicase DDX17 with RNA pri-18a-oligo1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 41 2 2
Unit cell lengths	62.504, 62.504, 231.196
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.545 - 1.700
R-factor	0.1738
Rwork	0.172
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	DDX17_rU10
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.690	1.700
Rmeas	0.168	3.717
Number of reflections	52488	2555
<I/σ(I)>	36.1
Completeness [%]	99.9
Redundancy	22.9
CC(1/2)	0.996	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M sodium acetate pH 4.6, 0.1M sodium chloride, 14% MDP, 10 mM CaCl2