6UV0

Crystal structure of apo core domain of RNA helicase DDX17

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.07218
Spacegroup name	P 21 21 21
Unit cell lengths	67.649, 97.811, 154.924
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.906 - 2.601
R-factor	0.216
Rwork	0.213
R-free	0.25950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Apo DDX17
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmeas	0.152	0.824
Number of reflections	32184	1564
<I/σ(I)>	42.8
Completeness [%]	99.7
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	24% PEG, 0.26M NaSCN