6UUK
Crystal structure of muramoyltetrapeptide carboxypeptidase from Oxalobacter formigenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-06 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 63 |
Unit cell lengths | 82.443, 82.443, 149.293 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.826 - 2.348 |
R-factor | 0.2093 |
Rwork | 0.204 |
R-free | 0.22930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zrs |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
High resolution limit [Å] | 2.348 | 6.370 | 2.348 |
Rmerge | 0.099 | 0.054 | 0.760 |
Rmeas | 0.107 | 0.058 | 0.854 |
Rpim | 0.040 | 0.021 | 0.379 |
Number of reflections | 23955 | 1225 | 1156 |
<I/σ(I)> | 6.5 | ||
Completeness [%] | 99.9 | 99.4 | 99.1 |
Redundancy | 6.5 | 6.8 | 4.4 |
CC(1/2) | 0.995 | 0.505 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1 M HEPES, 25% PEG3350, 0.2M sodium chloride |