6UUF
Crystal structure of a Nudix Hydrolase from M. Smegmatis, RenU
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-09 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.93927 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.198, 118.738, 36.432 |
Unit cell angles | 90.00, 92.32, 90.00 |
Refinement procedure
Resolution | 59.370 - 2.100 |
R-factor | 0.1828 |
Rwork | 0.180 |
R-free | 0.23110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hhj |
RMSD bond length | 0.014 |
RMSD bond angle | 1.601 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.370 | 10.000 | 2.140 |
High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
Rmerge | 0.072 | 0.051 | 0.177 |
Rmeas | 0.087 | 0.061 | 0.218 |
Rpim | 0.047 | 0.033 | 0.124 |
Number of reflections | 15812 | 861 | 633 |
<I/σ(I)> | 9 | ||
Completeness [%] | 93.6 | 99.7 | 72.3 |
Redundancy | 3.2 | 3.3 | 2.6 |
CC(1/2) | 0.994 | 0.961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1-1.4 M NaAc, 0.1 M BTP pH 7.5 |