6UU4
E. coli sigma-S transcription initiation complex with a 3-nt RNA ("old" crystal soaked with GTP and dinucleotide GpA for 30 minutes)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 132.143, 153.432, 230.958 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.975 - 4.305 |
Rwork | 0.304 |
R-free | 0.36370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ipl |
RMSD bond length | 0.006 |
RMSD bond angle | 1.349 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 4.560 |
High resolution limit [Å] | 4.300 | 4.300 |
Number of reflections | 32436 | 5091 |
<I/σ(I)> | 11.74 | 0.54 |
Completeness [%] | 99.6 | 98.6 |
Redundancy | 13.4 | 13.1 |
CC(1/2) | 0.999 | 0.220 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | PEG3350, sodium chloride, HEPES |