6UTO
Native E. coli Glyceraldehyde 3-phosphate dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-13 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 77.273, 186.709, 122.132 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.600 - 1.640 |
R-factor | 0.1661 |
Rwork | 0.166 |
R-free | 0.18860 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.040 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.670 |
High resolution limit [Å] | 1.640 | 1.640 |
Number of reflections | 1157097 | 107066 |
<I/σ(I)> | 44.7 | |
Completeness [%] | 99.6 | |
Redundancy | 11.6 | |
CC(1/2) | 0.860 | 0.860 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 5.5 mg/mL protein, 0.1 M HEPES pH 7.5 and 1.43 M sodium citrate |