6UTN
Native E. coli Glyceraldehyde 3-phosphate dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-02 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 77.673, 187.353, 121.673 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.840 - 1.790 |
R-factor | 0.1782 |
Rwork | 0.178 |
R-free | 0.19860 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.324 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.840 | 1.820 |
High resolution limit [Å] | 1.790 | 1.790 |
Number of reflections | 746389 | 83268 |
<I/σ(I)> | 37.7 | |
Completeness [%] | 99.8 | |
Redundancy | 9 | |
CC(1/2) | 0.870 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 5.5 mg/mL protein, 0.1 M HEPES pH 7.5 and 1.43 M sodium citrate |