6UTM

Native E. coli Glyceraldehyde 3-phosphate dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-02
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	77.912, 186.966, 121.751
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.740 - 2.140
R-factor	0.1613
Rwork	0.161
R-free	0.19900
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.034
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.740	2.170
High resolution limit [Å]	2.140	2.140
Number of reflections	48546	14278
<I/σ(I)>	31.3
Completeness [%]	97.9
Redundancy	3.4
CC(1/2)	0.860	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	5.5 mg/ mL protein, 0.1 M HEPES pH 7.5 and 1.43 M sodium citrate